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Greiner Bio-One开发了具有细胞排斥表面的CELLSTAR®细胞培养产品，用于3D培养。细胞排斥表面有效地防止细胞贴壁，从而可以促进三维球体的自发形成：圆底微孔板每个孔中的单个球状体或平底板中的多个球状体。对于高放大倍率下单个球状体的显微镜分析等应用，Greiner Bio-One还提供磁性3D细胞培养技术，能够满足高通量筛选对理想光学特性的需求。
两种产品线均可以使您使用快速简便的2D工作流程实现3D培养！
磁性3D细胞培养论文合集
磁性3D细胞培养protocol
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文献和实验| Year | Author/Title/Link | Reference | Application Area | Cell type |
|---|---|---|---|---|
| 2020 | Kiss et al. Cisplatin treatment induced interleukin 6 and 8 production alters lung adenocarcinoma cell migration in an oncogenic mutation dependent manner | Respir. Res. (2020) 21:120 | Lung cancer, cell migration, BiO Assay, wound healing, drug screening | Primary lung cancer, A549 and PC-9 human lung adenocarcinoma |
| 2020 | Souza-Araújo et al. Three-Dimensional Cell Culture Based on Magnetic Fields to Assemble Low-Grade Ovarian Carcinoma Cell Aggregates Containing Lymphocytes | Cells. 9(3): 635. 2020 | Ovarian Cancer | Patient cells Ovarian carcinoma Lymphocytes |
| 2019 | Urbanczyk et al. Controlled Heterotypic Pseudo-Islet Assembly of Human b-Cells and Human Umbilical Vein Endothelial Cells Using Magnetic Levitation | Tissue Engineering Part AVol. 26, No. 7-8 | Co-culture Tissue engineering |
Pancreatic ß-cells (EndoC-ßH3) Human umbilical vein endothelia cells (HUVECs) |
| 2019 | Leenhardt et al. Ultrasound-induced cavitation enhances the efficacy of Chemotherapy in a 3D Model of Pancreatic Ductal Adenocarcinoma with its microenvironment | Sci. Rep. 9, 18916 (2019) | Pancreatic Cancer | Murine embryonic fibroblasts (iMEF) KPC pancreatic ductal adenocarcinoma (PDA) DT66066 cells |
| 2019 | Antonino et al. Three-dimensional levitation culture improves in-vitro growth of secondary follicles in bovine model |
Reproductive BioMedicine. 38, 3, (2019) | Reproductive biology | Bovine secondary follicles Oocyte |
| 2019 | Bowser and Moore. Biofabrication of neural microphysiological systems using magnetic spheroid bioprinting | Biofabrication, 12, 1 (2019) | Tissue engineering Microfluidics |
Spinal cord cells |
| 2019 | A. Klinder et al. Comparison of different cell culture plates for the enrichment of non-adherent human mononuclear cells | Exp Ther Med. 2019 Mar; 17(3): 2004–2012 | Cell-Repellent | Peripheral blood mononuclear cells (PBMCs) Monocytes |
| 2019 | Mejía-Cruz et al. Generation of Organotypic Multicellular Spheres by Magnetic Levitation: Model for the Study of Human Hematopoietic Stem Cells Microenvironment |
International Journal of Stem Cells Vol. 12, No. 1, 2019 (2019) | Cancer Stem cells Organoids |
Human Hematopoietic Stem Cells Human bone marrow-mesenchymal stem cells Umbilical cord blood-hematopoietic stem cells Non-tumoral endothelial cell line |
| 2019 | Baillargeon et al. Automating a Magnetic 3D Spheroid Model Technology for High-Throughput Screening | SLAS Technology, Vol 24, Issue 4, 2019 (2019) | Screening Pancreatic Cancer Organoids |
Pancreatic cancer cells |
| 2019 | Ferreira et al. A magnetic three‐dimensional levitated primary cell culture system for the development of secretory salivary gland‐like organoids | J Tissue Eng Regen Med. 2019;13:495– 508 (2019) | Stem cells | Salivary gland derived cells |
3D Biosciences的磁力驱动3D细胞培养做一简要介绍：美国n3D Biosciences,Inc 以磁化细胞培养技术为基础，研发出一系列磁力驱动3D细胞培养产品。 磁化细胞培养技术：以NanoShuttle（一种生物相容性磁性纳米颗粒）为中心，将其加入到细胞或培养基中磁化细胞，然后再使用磁力驱动器，就可以让细胞进入磁悬浮状态或将细胞打印（printing）成具有结构和生物性能的典型3D模型（环状或微球状）。 对于96孔板试剂盒来说，磁力驱动器有两种：Dot磁力驱动器和Ring磁力驱动
的体外实验中模拟细胞在体内的行为，”3D Biomatrix公司的CEO Laura Schrader说。“传统2D细胞培养往往无法了解细胞在组织内的功能和应答反应，结果可能导致以2D细胞培养为基础的药物或生物学研究出现偏颇，并且也难以预测药物在体内的效果。而3D细胞培养可以为研究者们提供药物等更真实的早期信息，从而降低像市场推出新药的研究成本。” 近来3D细胞培养 产品如雨后春笋一般涌现出来，这也使越来越多的研究者们得以享受到这一技术带来的好处。那么我们要如何选择最适合自己的3D培养
未经过病毒测试。不过，我们已检测过多批产品，确定其不含乳酸脱氢酶增高病毒(LDEV)，而且我们的原料供应商Harlan在他们的无特定病原体级(SPF)动物检测清单中加入了LDEV检测项目，因此原料(小鼠)中也不含此病毒。此外，通过风险评估确定，RNA病毒无法在生产过程中存活下来。综合以上三点，我们基本可以确定本品不含LDEV。 冻融循环后，ECM Gel基质胶还适合进行3D细胞培养吗？ 我们不建议对ECM Gel基质胶产品进行解冻和再冷冻操作。可改将凝胶倒入平板，在2-8° C下储存。储存凝胶
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